日本一级电影有哪些

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      日本一级电影有哪些

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        MolluscDB

        Integrated genomics database for Mollusca

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        Select the Task for primer selection

        By location:

        Ref. Genome     

        Position     

        By genome

        sequence:

        General Primer Picking Conditions

        Primer Size
        PRIMER_MIN_SIZE (int)
        Minimum acceptable length of a primer. Must be greater than 0 and less than or equal to PRIMER_MAX_SIZE.
        Min
        PRIMER_OPT_SIZE (int)
        Optimum length (in bases) of a primer. Primer3 will attempt to pick primers close to this length.
        Opt
        PRIMER_MAX_SIZE (int)
        Maximum acceptable length (in bases) of a primer. Currently this parameter cannot be larger than 35. This limit is governed by maximum oligo size for which Primer3's melting-temperature is valid.
        Max
        Primer Tm
        PRIMER_MIN_TM (float)
        Minimum acceptable melting temperature (Celsius) for a primer oligo.
        Min
        PRIMER_OPT_TM (float)
        Optimum melting temperature (Celsius) for a primer. Primer3 will try to pick primers with melting temperatures are close to this temperature. The oligo melting temperature formula used can be specified by user. Please see PRIMER_TM_FORMULA for more information.
        Opt
        PRIMER_MAX_TM (float)
        Maximum acceptable melting temperature (Celsius) for a primer oligo.
        Max
        PRIMER_PAIR_MAX_DIFF_TM (float)
        Maximum acceptable (unsigned) difference between the melting temperatures of the left and right primers.
        Product Tm
        PRIMER_MIN_GC (float)
        Minimum allowable percentage of Gs and Cs in any primer.
        Min
        PRIMER_OPT_GC_PERCENT (float)
        Optimum GC percent. This parameter influences primer selection only if PRIMER_WT_GC_PERCENT_GT or PRIMER_WT_GC_PERCENT_LT are non-0.
        Opt
        PRIMER_MAX_GC (float)
        Maximum allowable percentage of Gs and Cs in any primer generated by Primer.
        Max
        Primer GC%
        PRIMER_MIN_GC (float)
        Minimum allowable percentage of Gs and Cs in any primer.
        Min
        PRIMER_OPT_GC_PERCENT (float)
        Optimum GC percent. This parameter influences primer selection only if PRIMER_WT_GC_PERCENT_GT or PRIMER_WT_GC_PERCENT_LT are non-0.
        Opt
        PRIMER_MAX_GC (float)
        Maximum allowable percentage of Gs and Cs in any primer generated by Primer.
        Max
        PRIMER_PRODUCT_SIZE_RANGE (size range list)
        The associated values specify the lengths of the product that the user wants the primers to create, and is a space separated list of elements of the form - where an - pair is a legal range of lengths for the product. For example, if one wants PCR products to be between 100 to 150 bases (inclusive) then one would set this parameter to 100-150. If one desires PCR products in either the range from 100 to 150 bases or in the range from 200 to 250 bases then one would set this parameter to 100-150 200-250. Primer3 favors product-size ranges to the left side of the parameter string. Primer3 will return legal primers pairs in the first range regardless the value of the objective function for pairs in subsequent ranges. Only if there are an insufficient number of primers in the first range will Primer3 return primers in a subsequent range. For those with primarily a computational background, the PCR product size is the size (in base pairs) of the DNA fragment that would be produced by the PCR reaction on the given sequence template. This would, of course, include the primers themselves.
        Product Size Ranges
        PRIMER_NUM_RETURN (int)
        The maximum number of primer (pairs) to return. Primer pairs returned are sorted by their "quality", in other words by the value of the objective function (where a lower number indicates a better primer pair). Caution: setting this parameter to a large value will increase running time.
        Number To Return
        PRIMER_MAX_END_STABILITY (float, 999.9999)
        The maximum stability for the last five 3' bases of a left or right primer. Bigger numbers mean more stable 3' ends. The value is the maximum delta G (kcal/mol) for duplex disruption for the five 3' bases as calculated using the nearest-neighbor parameter values specified by the option of PRIMER_TM_FORMULA For example if the table of thermodynamic parameters suggested by SantaLucia 1998, DOI:10.1073/pnas.95.4.1460 is used the deltaG values for the most stable and for the most labile 5mer duplex are 6.86 kcal/mol (GCGCG) and 0.86 kcal/mol (TATAT) respectively. If the table of thermodynamic parameters suggested by Breslauer et al. 1986, 10.1073/pnas.83.11.3746 is used the deltaG values for the most stable and for the most labile 5mer are 13.4 kcal/mol (GCGCG) and 4.6 kcal/mol (TATAC) respectively.
        Max 3' Stability
        Pick Primers Reset Form

        日本一级电影有哪些

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